How to view pollination pollen quality?

The quality of farmers buy pear pollen, determines the pear IVF success rates, will determine the pear fruit! How do you view the quality of pollination pollen?
Artificial fruit pollination pollen is active pollen vitality, but all need specific conditions in order to ensure its vitality in the processing, storage and transportation process, otherwise it will reduce the germination rate, and even death. In order to ensure the normal effect of artificial pollination of fruit trees, we should select the qualified products for artificial pollination. The pollen quality of fruit trees can be seen from the following two aspects!
Sensory quality detection
Tested by nasal olfactory visual.
The pollen into powder, no mildew, and has the unique color, smell varieties.
Pollen should be pure, no foreign body.
The pollen grains are allowed to have the original anther shell, and the pure pollen grains must pass through the 0.15mm screen.

Two, physiological index detection
Instrument and equipment
Medicine, medicinal peony cotton stick, tray balance, thermometer, incubator, beaker, crucible clamp, electric furnace, glass rod, glass slides, Petri dishes, microscope, dropper, test paper, PH bottle.
Experimental materials
Sucrose, agar, boric acid, hydrochloric acid, distilled water for chemical analysis.
Culture medium preparation
With 50ml of distilled water by adding 0.5 grams of agar 5g sucrose dissolved after heating, adding sucrose to be completely dissolved, adding 0.01% boric acid with hydrochloric acid pH modulation of 5.5-6.0 coated on glass slides.
The experiment steps
1, to medium temperature culture on a glass slide to room temperature after the trace pollen sprinkled gently on culture medium.
2, to the culture dish into the absorbent paper and pour distilled water will be sprinkled with moisture, pollen slides are placed in a Petri dish, with the lid, the Petri dish placed in constant temperature incubator, 25 C culture 4h.
3. The number of pollen cells and the number of germinated pollen cells were counted by microscope.
Germination percentage (%) = (Q/W) *100
Formula
Q - the number of germinated pollen cells observed;
W - the total number of pollen cells observed.

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